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Registros recuperados: 63
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Seedling production of Atlantic bluefin tuna (ABFT) Thunnus thynnus. The selfdott project ArchiMer
De La Gandara, Fernando; Mylonas, Constantinos C.; Coves, Denis; Ortega, Aurelio; Bridges, Christopher R.; Belmonte Rios, Antonio; Vassallo-agius, Robert; Papandroulakis, Nikos; Rosenfeld, Hanna; Tandler, A; Medina, Antonio; Demetrio, A; Corriero, Aldo; Fauvel, Christian; Falcon, Jack; Sveinsvoll, K; Ghysen, Alain; Deguara, S; Gordin, Hillel.
Tipo: Text
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00029/14015/11205.pdf
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Growth and reproductive performances of juveniles and sexually mature pollack Pollachius pollachius in different conditions ArchiMer
Omnes, Marie-helene; Severe, Armelle; Barone, Herve; Suquet, Marc; Buchet, Vincent; Le Roux, Annick; Gaignon, Jean-louis; Fostier, Alexis; Fauvel, Christian.
The pollack Pollachius pollachius is a commercially important fish, considered as a candidate for aquaculture in France. Rearing attempts are promising (Suquet et al., 1996), and more information is required about its reproduction, to contribute to a better management of breeders for this species. This study was designed to examine the first maturity in pollack and the effects of conditions such as origin and delayed reproduction season, on gonad maturation, reproductive and growth performances of adults.
Tipo: Text
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00002/11324/7861.pdf
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The Nme gene family in fish ArchiMer
Desvignes, T.; Fostier, A.; Fauvel, Christian; Bobe, J..
The Nme gene family, also known as Nm23 or NDPK, is a very ancient gene family that can be found in all kingdoms of life. In the late eighties, a gene of the Nme family, NME1, was identified as the first metastatic suppressor gene, resulting in a major interest for this family. Due to the complexity of the family, the need for a unified and evolutionary-supported gene nomenclature was recently stressed by the scientific community. Based on a complete evolutionary history study of the gene family in metazoans and vertebrates, a unified nomenclature was recently proposed and accepted by gene nomenclature consortia. In addition to its well-documented role in tumor metastasis, members of the Nme family are also involved in a wide variety of cellular and...
Tipo: Text Palavras-chave: Nm23; NDPK; RP2; Teleost; Oocyte; Ovary.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00133/24466/22504.pdf
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Sperm features of captive Atlantic bluefin tuna (Thunnus thynnus) ArchiMer
Suquet, Marc; Cosson, J.; De La Gandara, F.; Mylonas, C. C.; Papadaki, M.; Lallemant, S.; Fauvel, Christian.
P>The present study aimed to establish some basic characteristics of Atlantic bluefin tuna sperm from captive mature males, treated or untreated by gonadotropin releasing hormones agonist (GnRHa). Intratesticular milt was collected from treated and untreated fish (mean weight +/- SD: 122.9 +/- 29.2 kg, n = 21). There was no significant effect of GnRHa treatment on GSI (1.33 +/- 0.70%, n = 21) or on sperm concentration (3.8 +/- 1.3 x 1010 spermatozoa ml-1, n = 21) estimated by optical density at 260 nm. Similarly, the percentage of motile spermatozoa measured at 30 s post activation: activating medium (AM seawater containing 10 mg ml-1 BSA) was not significantly different between control and GnRHa implanted males. On the other hand, a significantly...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12645/9722.pdf
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X-Linked Retinitis Pigmentosa 2 Is a Novel Maternal-Effect Gene Required for Left-Right Asymmetry in Zebrafish ArchiMer
Desvignes, Thomas; Nguyen, Thaovi; Chesnel, Franck; Bouleau, Aurelien; Fauvel, Christian; Bobe, Julien.
Retinitis pigmentosa 2 (RP2) gene is responsible for up to 20% of X-linked retinitis pigmentosa, a severe heterogeneous genetic disorder resulting in progressive retinal degeneration in humans. In vertebrates, several bodies of evidence have clearly established the role of Rp2 protein in cilia genesis and/or function. Unexpectedly, some observations in zebrafish have suggested the oocyte-predominant expression of the rp2 gene, a typical feature of maternal-effect genes. In the present study, we investigate the maternal inheritance of rp2 gene products in zebrafish eggs in order to address whether rp2 could be a novel maternal-effect gene required for normal development. Although both rp2 mRNA and corresponding protein are expressed during oogenesis, rp2...
Tipo: Text Palavras-chave: Developmental biology; Egg developmental competence; Egg quality; Fish; Fish reproduction; Maternal-effect gene; Ndpk; Nme10; Oocyte; Oocyte-specific; Ovum; Teleost; Zygote.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00280/39099/37660.pdf
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Maternally Inherited npm2 mRNA Is Crucial for Egg Developmental Competence in Zebrafish ArchiMer
Bouleau, Aurelien; Desvignes, Thomas; Traverso, Juan Martin; Nguyen, Thaovi; Chesnel, Franck; Fauvel, Christian; Bobe, Julien.
The molecular mechanisms underlying and determining egg developmental competence remain poorly understood in vertebrates. Nucleoplasmin (Npm2) is one of the few known maternal effect genes in mammals, but this maternal effect has never been demonstrated in nonmammalian species. A link between developmental competence and the abundance of npm2 maternal mRNA in the egg was previously established using a teleost fish model for egg quality. The importance of maternal npm2 mRNA for egg developmental competence remains unknown in any vertebrate species. In the present study, we aimed to characterize the contribution of npm2 maternal mRNA to early developmental success in zebrafish using a knockdown strategy. We report here the oocyte- specific expression of npm2...
Tipo: Text Palavras-chave: Egg quality; Fish; Maternal effect gene; Maternal-to-zygotic transition; Nucleoplasmin; Oocyte developmental competence; Teleost; Zygotic genome activation.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00211/32234/30685.pdf
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Human chorionic gonadotropin induces spermatogenesis and spermiation in 1-year-old European sea bass (Dicentrarchus labrax): Assessment of sperm quality ArchiMer
Schiavone, R; Zilli, L; Vilella, S; Fauvel, Christian.
The aims of the present study were (a) to compare sperm quality (percentage of motile spermatozoa, motility duration, density and fertility after cryopreservation) between precocious and normally maturing male European sea bass Dicentrarchus labrax, (b) to examine the potential of human chorionic gonadotropin (hCG) to increase spermiation in precocious males and (c) to examine the potential of hCG to induce spermatogenesis and spermiation in non-precocious 1-year-old males. One hundred precocious and 100 non-precocious fish were each randomly divided in two groups each: control (precocious saline-treated and non precocious saline-treated) and treated (precocious hCG-treated and non precocious hCG-treated). Treated groups were administered weekly with 1000...
Tipo: Text Palavras-chave: Dicentrarchus labrax; HCG; European sea bass; Spermiation; Spermatogenesis.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1815.pdf
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Evaluation of fish sperm quality ArchiMer
Fauvel, Christian; Suquet, Marc; Cosson, J..
P>The development of cheaper technical tools and the availability of well established assay kits provide today the spermatologists with several devices for objective and quantifying assessment of sperm quality. The present paper reviews modern methods on sperm concentration assessment, morphometry, motility processes through flagellum studies, motility objective assessment by computer assisted sperm analysis (CASA), sperm integrity and energetic state measurements.
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12642/13921.pdf
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Diversify: results from the first year of wreckfish (Polyprion americanus) culture ArchiMer
Peleteiro, J.b.; Linares, F; Vilar, A; Fauvel, Christian; Duncan, N; Rodriguez, C; Izquierdo, M; Mylonas, C.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00307/41772/40987.pdf
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Effect of stripping frequency on turbot spawn quality. ArchiMer
Fauvel, Christian; Omnes, Marie-helene; Suquet, Marc; Normant, Yvon.
In production operations the application of daily examination of each female of broodstock should be a determinant progress in hatchery management in terms of fry output. The eggs expelled shortly after ovulation by means of a systematic fivefold a week stripping attempt of females, presented a high viability rate (72%) compared to that of eggs collected twice a week (46%) whereas the treatments did not have any effect on female fecundity. Increase of the stripping rhythm brought the hatching rates and broodstock fertilities respectively from 11% to 35% and from 20,000 to 65,000 larvae/kg of spawning female. The enhancement of egg quality and of the resulting larva production is discussed.
Tipo: Text
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00170/28129/26346.pdf
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First summary of studies carried out on the grey mullet (Mugil cephalus) in the EU project: diversify ArchiMer
Koven, W; Rosenfeld, H; Fauvel, Christian; Nixon, O; Meiri-ashkenazi, I; Bitan, A; Robles, R; Kotzamanis, Y; Shafran, G; Papaioannou, N.
The deliverable (D7.1) of WP7 “Establishment of a Computer Assisted Sperm Analysis (CASA) for the evaluation of grey mullet sperm” has been submitted. Preliminary analyses show that mullet sperm motility pattern is not similar to that of sea bass (Dicentrarchus labrax) or rainbow trout (Oncorhynchus mykiss) in terms of velocity as well as motility duration (ca 1 min; Fig. 1a). Our preliminary results, indicate that the major bio-technical settings, dilution of sperm, quality of activation and mastering of video recordings have been determined and can now be applied to experimental protocols. Since fish sperm quality is usually assessed through subjective observation, in terms of motility classes and duration, statistical analysis becomes less precise. The...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00307/41776/40991.pdf
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Sperm features in turbot (Scophthalmus maximus): a comparison with other freshwater and marine fish species ArchiMer
Suquet, Marc; Billard, Roland; Cosson, Jacky; Dorange, Germaine; Chauvaud, Laurent; Mugnier, Chantal; Fauvel, Christian.
This paper presents some features of turbot sperm and compares them with data reported in the literature on other freshwater and marine fish. In captivity, turbot spermiation period lasts 6 months. When compared to other fish species, turbot males are characterized by low values of maximum gonadosomatic index (0.6-0.8%) and are poor sperm producers in terms of volume (0.2-2.2 ml), concentration (0.7-11.0 x 10(9) spermatozoa/ml) and total sperm number released per stripping (0.2-12.0 x 10(9) spermatozoa). Compared to most freshwater species, the duration of cell movement is long (1-17 min). The spermatozoon of turbot can be considered to be of a primitive type. A very high protein content (8.8 mg/ml) is found in the seminal fluid of turbot. Increasing...
Tipo: Text Palavras-chave: Scophthalmus maximus; Turbot; Biologie du sperme; Liquide séminal; Mobilité du spermatozoïde; Morphologie du spermatozoïde; Poisson marin; Scophthalmus maximus; Turbot; Sperm biology; Seminal fluid; Sperm motility; Sperm morphology; Marine fish.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00188/29893/28334.pdf
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Homologous ELISA procedure for the determination of penaeid shrimp vitellogenin ArchiMer
Mendoza, Roberto; Guillaume, Jean-charles; Fauvel, Christian.
Vitellogenin (VTG) was isolated from the haemolymph of Penaeus vannamei by a three step procedure including ultracentrifugation, gel filtration and ion exchange chromatography. VTG was used to raise polyclonal antibodies that were purified by ion exchange chromatography. A two step competitive assay was developed in which VTG could be quantitated by its capacity to inhibit the binding of antibody to the VTG previously adsorbed onto a solid phase. Sensitivity from equilibrium and from non-equilibrium assays was 41 and 2.3 ng/ml respectively. Estimates of within-assay and between-assay variabilities of standard curves were 4.2 and 9.6% respectively. The inhibition curves for dilutions of haemolymph from vitellogenic females, egg yolk extracts and purified...
Tipo: Text Palavras-chave: Pénéidé; Vitellogénine; Vitelline; Immuno-essai (ELISA); Anticorps; Penaeid; Vitellogenin; Vitellin; Immunoassay (ELISA); Antibody.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00188/29894/28333.pdf
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Description of hake (Merlucius merlucius) spermatozoa: flagellar wave characteristics and motility parameters in various situations ArchiMer
Cosson, J.; Groison,; Fauvel, Christian; Suquet, Marc.
P>The objective of this study was to complement published hake (Merlucius merlucius) sperm motility characteristics by obtention of data more specifically dependent on flagella: beat frequency, flagellar wave amplitude, wave length, number of waves along the length of the flagellum and curvature of the wave. The changes of these parameters are described in relation to the time period elapsed since activation are described using high resolution video images of flagella while quantifying the progression during the swimming period from the activity initiation to full cessation of movement several minutes later. The main characteristic of the swimming period of hake spermatozoa shows a progressive decrease of forward displacement resulting from a cumulative...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12643/9586.pdf
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Pollack (Pollachius pollachius): Acquisition of biologic data in a breeding purpose ArchiMer
Buchet, Vincent; Suquet, Marc; Omnes, Marie-helene; Petton, Bruno; Normant, Yvon; Severe, Armelle; Quemener, Loic; Barone, Herve; Fauvel, Christian; Gaignon, Jean-louis.
The fishing of pollack which concerns essentially, France, United Kingdom and Norway, and at a lower level Ireland and Denmark, is situated between 10 and 15000 tons a year. In 1996, France was the first producer of Pollack. Since 1980, a decrease of production is observed in Europe. In France, landing reached 3000t. in 1998 (Suquet, 2001). However, the demand remaining strong, aquaculture could be a solution to satisfy the deficit between the French production and the domestic demand.
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Ano: 2002 URL: http://archimer.ifremer.fr/doc/00170/28144/26365.pdf
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Nme protein family evolutionary history, a vertebrate perspective ArchiMer
Desvignes, Thomas; Pontarotti, Pierre; Fauvel, Christian; Bobe, Julien.
Background: The Nme family, previously known as Nm23 or NDPK, is involved in various molecular processes including tumor metastasis and some members of the family, but not all, exhibit a Nucleoside Diphosphate Kinase (NDPK) activity. Ten genes are known in humans, in which some members have been extensively studied. In non-mammalian species, the Nme protein family has received, in contrast, far less attention. The picture of the vertebrate Nme family remains thus incomplete and orthology relationships with mammalian counterparts were only partially characterized. The present study therefore aimed at characterizing the Nme gene repertoire in vertebrates with special interest for teleosts, and providing a comprehensive overview of the Nme gene family...
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6947.pdf
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La domestication des poissons : le cas du thon rouge ArchiMer
Fauvel, Christian; Suquet, Marc.
Bluefin tuna shows adaptative physiology features allowing life in a wide range of physical and climatic parameters like temperature, salinity and hydrostatic pressure, hence the species fulfil the main basic requirements for progressive domestication and rearing in captivity. The high mortalities recorded after handling and the losses observed after abrupt variations of environment in the cages, emphasised the extreme sensitivity to stress of tuna. As consequences, the progresses about the control of tuna biological cycle are very slow due to limited handling possibilities and tuna aquaculture is restricted to fattening of wild fish. At this time, bluefin tuna production progresses without domestication of the species.
Tipo: Text
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00272/38283/36473.pdf
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Effect of age and environmental conditions on the movement characteristics of Pacific oyster (Crassostrea gigas) trochophores ArchiMer
Suquet, Marc; Rimond, Flore; Cosson, J.; Wilson-leedy, J.; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Fauvel, Christian.
The movement characteristics of Pacific oyster trochophores have received very little coverage in the scientific literature. Described here are not only changes in the swimming characteristics of Crassostrea gigas trochophores (size: 53-77 mu m) in relation to time after fertilization and to inter-female variation but also the effects of salinity and pH on movement characteristics. The percentage of motile trochophores was measured on images obtained through a dissecting microscope and the Velocity Average Path (VAP) was assessed using a Computer Assisted Sperm Analysis (CASA) system. At 20 degrees C, the first movements of the trochophores were observed at 6.5 h post-fertilization. The mean (+/- SD) percentage of swimming trochophores and the VAP observed...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26786/24940.pdf
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Egg production in the turbot (Scophthalmus maximus) in relation to broodstock management ArchiMer
Omnes, Marie-helene; Normant, Yvon; Suquet, Marc; Fauvel, Christian.
Egg production data from individual hand stripped female turbot were collected and analysed over several consecutive spawning seasons, on natural or shifted conditions. Egg quantity, egg viability and number of spawns were examined from one spawning season to the other, and inside the spawning season. These parameters seemed to increase from a year to the next one, except for the oldest fishes. Spawns were spread over a three-month period and occurred at the same time in the period in successive seasons for most females. Egg production seemed related to the feeding level and the earliness of spawning season appeared depended on the growth rate. Without sand substrate in tanks, injuries could increase therefore no significant differences were observed in...
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/00199/31018/29423.pdf
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Cryopreservation of sperm in marine fish ArchiMer
Suquet, Marc; Dreanno, Catherine; Fauvel, Christian; Cosson, J; Billard, R.
Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results....
Tipo: Text Palavras-chave: DMSO; Insemination; Fertilization; Cryopreservation; Fish sperm.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-611.pdf
Registros recuperados: 63
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